Abstract: Problem statement: No specific antiviral therapy is currently available despite an emergence and resurgence of Japanese encephalitis in South-East Asian Countries. There are only few recent studies, which were aimed to treat Japanese encephalitis with newer drugs. There is thus a real need for study on antiviral agents that can reduce the toll of death and neurological sequelae resulting from infection with this virus. Approach: Optimum dilution of the JE virus was determined which could produce significant number of pocks on Chorioallantoic Membrane (CAM). Then ultradiluted belladonna preparations were used to see their inhibitory action on JE virus infection in CAM. Results: Ultradiluted belladonna showed significantly decreased pock count in CAM in comparison to JE virus control. Conclusion: Ultradiluted belladonna could inhibit JE virus infection in CAM, which may be mediated through glycosidase inhibitory role of calystegines present in belladonna. Key words: Japanese Encephalitis (JE), Chorioallantoic Membrane (CAM), pock, belladonna
Abstract BACKGROUND: Leptospirosis is a zoonotic disease of major importance in the tropics where the incidence peaks in rainy seasons. Natural disasters represent a big challenge to Leptospirosis prevention strategies especially in endemic regions. Vaccination is an effective option but of reduced effectiveness in emergency situations.
Homeoprophylactic interventions might help to control epidemics by using highly-diluted pathogens to induce protection in a short time scale. We report the results of a very large-scale homeoprophylaxis (HP) intervention against Leptospirosis in a dangerous epidemic situation in three provinces of Cuba in 2007.
METHODS:Forecast models were used to estimate possible trends of disease incidence. A homeoprophylactic formulation was prepared from dilutions of four circulating strains of Leptospirosis. This formulation was administered orally to 2.3 million persons at high risk in an epidemic in a region affected by natural disasters. The data from surveillance were used to measure the impact of the intervention by comparing with historical trends and non-intervention regions.
RESULTS:After the homeoprophylactic intervention a significant decrease of the disease incidence was observed in the intervention regions. No such modifications were observed in non-intervention regions. In the intervention region the incidence of Leptospirosis fell below the historic median. This observation was independent of rainfall.
CONCLUSIONS:The homeoprophylactic approach was associated with a large reduction of disease incidence and control of the epidemic. The results suggest the use of HP as a feasible tool for epidemic control, further research is warranted. 2010 Elsevier Ltd. All rights reserved.
Use of the nosode Meningococcinum as a preventive against meningitis
Castro, D. & Nogueira, G. G. (1975). Use of the nosode Meningococcinum as a preventive against meningitis.
Journal of the American Institute of Homœopathy, 1975 Dec 68(4), 211-219.
Abstract: A campaign was made in Guaratingueta, a city in the State of San Paulo, Brazil, in August, 1974. 18,640 persons were immunised, with 6,340 children not being covered. The product used for immunisation was the nosode Meningococcinum 10CH, in a single dose.
For the analysis of the results, a comparison was made of the occurrence of meningitis during the months from August 1974 up to February 1975, in the immunised and non-immunised groups of the city. For the statistical analysis the Pearson’s X2 test was employed.
The following results were obtained: 18,640 protected homoeopathically 4 cases 6,340 not protected 32 cases. Based on the attack rate in the unprotected group, 94 cases would have been expected in the homoeopathically protected group. There were only 4, showing that the homœopathic option was 95% effective.
Menigococcinum: Its protective effect against meningococcal disease
Mroninski C, Adriano E & Mattos G (1998/99)
Homœopathic Links, Vol 14 Winter 2001, 230-234
Abstract: A report on a Government funded initiative in the city of Blumenau in the north of Santa Catarina State in Brazil conducted by senior medical staff. The study relates to the use of the nosode Meningococcinum 30CH on 65,826 people from 0 – 20 years of age in 1998 with a control group of 23,532.
The results were statistically significant, offering a protection against Meningococcal Disease of 95% in six months and 91% in a year.
Credentials of the authors: Cleber R.L. MRONINSKI Specialist Physician in Public Health, Professor of the Medical Clinic Department at University Foundation in Blumenau Edson Jose ADRIANO Specialist Physician in Work Medicine, Health City Secretary in Blumenau Gerson MATTOS Homœopath, Specialist in Community and General Medicine, Professor of the Medical Clinic at the University Foundation in Blumenau Rua Waldir Medeiros 122 Bairro Vellha Blumenau 89042-340 Brazil
Antimalarial potential of China 30 and Chelidonium 30 in combination therapy against lethal rodent malaria parasite: Plasmodium berghei
Abstract: Homeopathy is a therapeutic method based on the application of similia principle, utilizing ultra-low doses of medicinal substances made from natural products. The present study has been designed to evaluate the efficacy of Cinchona officinalis (Chin.) 30C and Chelidonium majus (Chel.) 30C in combination therapy against lethal murine malaria.
Five groups having twelve BALB/c mice each were administered orally with 0.2 ml/mouse/day of different drugs, and their antimalarial potential was evaluated by Peter’s 4-day test.
The combination of Chin. 30 and Chel. 30 exhibited complete parasite clearance by the 28th day post-inoculation which was similar to the positive control [artesunate (4 mg/kg)+sulphadoxine-primethamine (1.2 mg/kg)] group.
Both the groups exhibited enhanced mean survival time (MST) 28±0 days,whereas, the mice of infected control group survived up to 7.6±0.4 days only. The preventive and curative activities of the combination in comparison to the positive controls [pyrimethamine (1.2 mg/Kg) and chloroquine (20 mg/Kg), respectively] were also evaluated.
The combination had a significant preventive activity (p<0.0005), with 89.2% chemosuppression which was higher than the standard drug, pyrimethamine (83.8%). It also showed a moderate curative activity with complete clearance of parasite in 50% of surviving mice, and enhancing the MST of mice up to 26.8±2.8 days.
These findings point to the significant antiplasmodial efficacy of the combination of these homeopathic drugs against Plasmodium berghei.
Resistance to antibiotics is a major public health concern worldwide. New treatment options are needed and homeopathy is one such option. We sought to assess the effect of the homeopathic medicine Belladonna (Bell) and a nosode (biotherapy) prepared from a multi-drug resistant bacterial species, methicillin-resistant Staphylococcus aureus (MRSA), on the same bacterium.
METHODS:Bell and MRSA nosode were prepared in 6cH and 30cH potencies in 30% alcohol and sterile water, according to the Brazilian Homeopathic Pharmacopeia and tested on MRSA National Collection of Type Cultures (NCTC) 10442. We assessed in vitro bacterial growth, deoxyribonuclease (DNAase) and hemolysin activity, and in vitro bacterial growth in combination with oxacillin (minimum inhibitory concentration - MIC). All values were compared to control: 30% alcohol and water.
RESULTS:In vitro growth of MRSA was statistically significantly inhibited in the presence of Bell and nosode 6cH and 30cH compared to controls (p < 0.0001); and with combination of Bell or nosode 6cH and 30cH and oxacillin (p < 0.001). Bell 30cH and nosode 6cH and 30cH significantly decreased bacterial DNAse production (p < 0.001) and reduced red blood cell lysis.
CONCLUSIONS:Cultures of MRSA treated with Belladonna or MRSA nosode exhibited reduced growth in vitro, reduced enzymatic activity and became more vulnerable to the action of the antibiotic oxacillin. Further studies are needed on the biomolecular basis of these effects.